Journal: PLoS ONE

Article Title: Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary Bypass

doi: 10.1371/journal.pone.0105759

Figure Lengend Snippet: Bar graph illustrating the change in global χ2 value by the addition of post-operative GDF-15 and/or NGAL to post-operative eGFR. All models were significantly associated with AKI (p<0.05). * = p<0.05 vs eGFR model. § = p = 0.06 vs eGFR + NGAL model.

Article Snippet: Plasma GDF-15 concentrations were measured by quantitative sandwich enzyme immunoassay (Human GDF-15, Quantikine, R&D Systems Europe, Lille, France) with a linear range from 200 to 50,000 ng/L.

Techniques:

Journal: Oncotarget

Article Title: MP0250, a VEGF and HGF neutralizing DARPin ® molecule shows high anti-tumor efficacy in mouse xenograft and patient-derived tumor models

doi: 10.18632/oncotarget.21738

Figure Lengend Snippet: Model of MP0250, which is composed of two human serum albumin (HSA) DARPin ® molecules flanking a VEGF and a HGF binding DARPin ® molecule ( A ). The binding of MP0250 to VEGF was determined by quantification of free hVEGF in solution after addition of increasing concentrations of MP0250 ( B ). Inhibition of VEGF-induced HUVEC proliferation was analyzed in the absence (grey triangle) or presence of increasing concentrations of MP0250. Cell growth was quantified by OD measurement representing BrdU uptake. Error bars indicate the standard deviation of independent duplicates ( C ). Competition of binding of VEGF-A to sVEGFR2-Fc ( D ) and sVEGFR1-Fc ( E ) in the presence of increasing concentrations of MP0250 (filled diamond). As a control, a non-binding DARPin ® molecule was titrated (filled grey circle).The HTRF signal was detected. Error bars indicate the standard deviation of independent triplicates (1d, 1e). Inhibition of cMET-phosphorylation in A549 cells by MP0250. Inhibition of phosphorylation as measured by ELISA measurement (OD450-620) versus the concentration of the inhibitor ( F ). Inhibition of U87MG proliferation by MP0250; error bars indicate the standard deviation of independent duplicates ( G ). Dashed black lines in the figures indicate IC50s. Data shown in the figure represents one experiment out of independent experiments as outlined in the Materials and Methods section.

Article Snippet: Human VEGF Quantikine and P-cMET ELISAs were from RnDsystems.

Techniques: Binding Assay, Inhibition, Standard Deviation, Control, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Oncotarget

Article Title: MP0250, a VEGF and HGF neutralizing DARPin ® molecule shows high anti-tumor efficacy in mouse xenograft and patient-derived tumor models

doi: 10.18632/oncotarget.21738

Figure Lengend Snippet: Tumor growth inhibition in the A673 rhabdomyosarcoma xenograft model ( A, B ) and the U87MG glioblastoma model ( C, D ). Figures and show the anti-tumor response to MP0250, the anti-HGF DARPin ® molecule and the anti-VEGF DARPin ® molecule. Figure and show a dose response of MP0250. Tumor growth is plotted as mean +/- SEM.

Article Snippet: Human VEGF Quantikine and P-cMET ELISAs were from RnDsystems.

Techniques: Inhibition

Journal: Oncotarget

Article Title: MP0250, a VEGF and HGF neutralizing DARPin ® molecule shows high anti-tumor efficacy in mouse xenograft and patient-derived tumor models

doi: 10.18632/oncotarget.21738

Figure Lengend Snippet: Tumor growth inhibition in the orthotopic renal cancer model (RENCA-LN model) ( A, B ) and the MC38 colorectal cancer model ( C, D ). Luciferase-transfected RENCA cells were orthotopically implanted into the left kidney of BalbB mice. Tumor growth was monitored by detection of luciferase activity in vivo during the study (Figure ) and determination of tumor volume at the end of the study (Figure ). MP0250 was compared to sorafenib at doses indicated in the figures. Figure shows the time course of the anti-tumor response to MP0250 and the HGF inhibitor and the VEGF inhibitor. Figure shows the tumor volumes at the end of the study. ( E ) shows the anti-angiogenic effect of the compounds in the MC38 effect demonstrated by immuno-histochemistry for CD31. Tumor growth is plotted as mean +/− SEM.

Article Snippet: Human VEGF Quantikine and P-cMET ELISAs were from RnDsystems.

Techniques: Inhibition, Luciferase, Transfection, Activity Assay, In Vivo, Immunohistochemistry

Journal: Pediatrics and neonatology

Article Title: The significance of serum and fecal levels of interleukin-6 and interleukin-8 in hospitalized children with acute rotavirus and norovirus gastroenteritis.

doi: 10.1016/j.pedneo.2013.05.008

Figure Lengend Snippet: Figure 1 Serum (A) interleukin-6 (IL-6) and (B) interleukin-8 (IL-8) levels in children with rotavirus, norovirus, bacterial gastroenteritis, and in healthy controls. The median with interquartile range (IQR) are shown in each group. p value for comparisons by Kruskal-Wallis test; *p < 0.05, by Dunn’s mul- tiple comparison test between groups.

Article Snippet: IL-6 and IL-8 levels were measured with a Quantikine Human Interleukin Immuno-assay (R&D Systems, Minneapolis, MN, USA) using the principle of quantitative sandwich enzyme immuno-assay with monoclonal antibodies specific for IL-6 and IL-8.

Techniques: Comparison